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Changes in types of muscle fibers induced by transcutaneous electrical stimulation of the diaphragm of rats BJMBR
Costa,D.; Cancelliero,K.M.; Campos,G.E.R.; Salvini,T.F.; da Silva,C.A..
The objective of the present study was to assess the effect of transcutaneous electrical diaphragmatic stimulation (TEDS) on different types of diaphragm muscle fibers. Male Wistar rats (8-12 weeks old) were divided into 2 experimental groups (N = 8 in each group): 1) control, 2) animals submitted to TEDS [frequency = 50 Hz; T ON/T OFF (contraction/relaxation time) = 2/2 s; pulse duration = 0.4 ms, intensity = 5 mA with a 1 mA increase every 3 min for 20 min] for 7 days. After completing this treatment period, the I, IIA, IIB, and IID diaphragm muscle fibers were identified using the mATPase technique. Statistical analysis consisted of the normality, homoscedasticity and t-tests (P < 0.05). There was a 19.6% (P < 0.05) reduction in the number of type...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Diaphragm; Transcutaneous electrical stimulation; Muscle fiber.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900011
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Rat hindlimb joint immobilization with acrylic resin orthoses BJMBR
da Silva,C.A.; Guirro,R.R.J.; Polacow,M.L.O.; Cancelliero,K.M.; Durigan,J.L.Q..
The objective of the present study was to propose an orthosis of light material that would be functional for the animal and that would maintain only the ankle joint immobilized. Male Wistar rats (3 to 4 months old, 250-300 g) were divided into 2 groups (N = 6): control and immobilized for 7 days. Rats were anesthetized with sodium pentobarbital (40 mg/kg weight) and the left hindlimb was immobilized with the orthoses composed of acrylic resin model, abdominal belt and lateral supports. The following analyses were performed: glycogen content of the soleus, extensor digitorum longus, white gastrocnemius, red gastrocnemius, and tibialis anterior muscles by the phenol sulfuric method, and the weight, fiber area and intramuscular connective tissue of the soleus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Hypotrophy; Joint; Orthoses; Physical therapy.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700016
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